Adapting the brood frame
Using the Jenter Kit
An empty brood frame was adapted to take the Jenter cage. Two vertical wooden struts were fixed to the frame so that the kit would push-fit in place. (A second such frame was also made for use later in the operation).

To prepare the frame for use the central part (where the Jenter would eventually go) was fitted with a push-fit small frame containing worker foundation. The two outside areas were left empty so that the bees would build drone comb.
This frame was place in frame position 4 in the chosen colony in the third week of March so that drones would be available at the end of April.

The Jenter Cage

In the third week in June this special brood frame was removed and the central mini frame, now containing worker brood, was exchanged for the Jenter cage. The frame was replaced in the hive for the next 24 hours so that the bees would become familiar with the Jenter cage.
The mini frame with the worker brood was placed in the second brood frame (prepared as above) and placed at the edge of the brood nest (frame 7).

Introducing the Queen

The next day at 6 pm the queen mother was found and sealed in the Jenter cage (day 0).
(It is important that there are no workers in the cage otherwise the queen could be attacked and killed. It is difficult to understand why the workers would want to attack their own queen but this has been learnt by experience.)
Exactly 24 hours later again at 6 pm (day 1) the queen was removed after laying up the kit with eggs. The frame was replaced in the brood nest and left for 3 days and overnight
Jenter queen cell
frame with replacable insert
Jenter cage in position
Transferring the royal larvae

At 10 am (day 4) the Jenter cells containing the royal larvae were removed and fitted to the plastic plugs on the cell bar frame. This was then immediately replaced in the Harden box and left for 10 days. A check was made after 5 days to ascertain if indeed any queen cells had been formed. A check was also made on the open brood frame just in case queen cells had been started here. In this case only 5 queens cells were formed on the bottom cell bar, one of which is shown in the photo on the left.
Once sealed the queen cells can be fitted with cell cages just in case the bees decide to tear them down.

Making up the nucs

On day 12 5 three frame nucs were made up. Each nuc consisting of one frame of sealed brood sandwiched between two frames of pollen. A queen cell was introduced to each nuc on the 14th day; the queen emerging two days later (day 16).

The overall timetable

A timetable summarizes the method as a pop-up
jenter eggs
Jenter larvae
Larvae seen on the 4th day in the brown Jenter cells just before insertion into the cell holders.
The tiny drops of royal jelly in each cell are reflecting the light.
Eggs seen standing upright in the Jenter cells after
the removal of the queen after 24 hours.
The dark cells are the removable cell plugs.
Next page: The Nucs
A nicely formed queen cell seen
on the cell bar frame on day 10
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